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epcam antibody  (Thermo Fisher)


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    Thermo Fisher epcam antibody
    (A) Characterization by NTA of purified exosomes derived from the MCF7 breast cancer cell line. (B) Cryo-TEM images (i) and (ii) of purified exosomes at an acceleration voltage of 200 kV. (C) Confocal microscopy images and (D) flow cytometry study for the (i) MCF7 breast cancer cell line and (ii) their exosomes covalently immobilized on MPs. For confocal microscopy, DNA appears <t>blue,</t> <t>magnetic</t> particles in green color, while the exosome protein membrane in red color. For flow cytometry, in all cases, the negative controls (obtained with incubation with the secondary antimouse-Cy5) are shown in blue, while the positive CD326 and CD81 samples obtained with the incubation with the <t>antiCD326</t> and antiCD81 primary antibodies, are shown in yellow and red, respectively. The negative control obtained with the exosomes-modified MPs without any labeling is shown in gray in panel D (ii).
    Epcam Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/epcam antibody/product/Thermo Fisher
    Average 94 stars, based on 69 article reviews
    epcam antibody - by Bioz Stars, 2026-03
    94/100 stars

    Images

    1) Product Images from "Electrochemical Genosensing of Overexpressed GAPDH Transcripts in Breast Cancer Exosomes"

    Article Title: Electrochemical Genosensing of Overexpressed GAPDH Transcripts in Breast Cancer Exosomes

    Journal: Analytical Chemistry

    doi: 10.1021/acs.analchem.2c04773

    (A) Characterization by NTA of purified exosomes derived from the MCF7 breast cancer cell line. (B) Cryo-TEM images (i) and (ii) of purified exosomes at an acceleration voltage of 200 kV. (C) Confocal microscopy images and (D) flow cytometry study for the (i) MCF7 breast cancer cell line and (ii) their exosomes covalently immobilized on MPs. For confocal microscopy, DNA appears blue, magnetic particles in green color, while the exosome protein membrane in red color. For flow cytometry, in all cases, the negative controls (obtained with incubation with the secondary antimouse-Cy5) are shown in blue, while the positive CD326 and CD81 samples obtained with the incubation with the antiCD326 and antiCD81 primary antibodies, are shown in yellow and red, respectively. The negative control obtained with the exosomes-modified MPs without any labeling is shown in gray in panel D (ii).
    Figure Legend Snippet: (A) Characterization by NTA of purified exosomes derived from the MCF7 breast cancer cell line. (B) Cryo-TEM images (i) and (ii) of purified exosomes at an acceleration voltage of 200 kV. (C) Confocal microscopy images and (D) flow cytometry study for the (i) MCF7 breast cancer cell line and (ii) their exosomes covalently immobilized on MPs. For confocal microscopy, DNA appears blue, magnetic particles in green color, while the exosome protein membrane in red color. For flow cytometry, in all cases, the negative controls (obtained with incubation with the secondary antimouse-Cy5) are shown in blue, while the positive CD326 and CD81 samples obtained with the incubation with the antiCD326 and antiCD81 primary antibodies, are shown in yellow and red, respectively. The negative control obtained with the exosomes-modified MPs without any labeling is shown in gray in panel D (ii).

    Techniques Used: Purification, Derivative Assay, Confocal Microscopy, Flow Cytometry, Incubation, Negative Control, Modification, Labeling

    Electrochemical genosensing of GAPDH transcripts from (A) MCF7 cells ranging from 50 to 5000 cells mL –1 and (B) their exosomes ranging from 100 to 4.0 × 10 4 exosomes μL –1 , according to NTA counting. In all cases, the cells and exosomes were lysed preconcentrated by IMS using antiCD81-MPs (▲) and antiCD326-MPs (●), followed by double-tagging RT-PCR on poly(dT)-MPs. The error bars show the standard deviation for n = 3.
    Figure Legend Snippet: Electrochemical genosensing of GAPDH transcripts from (A) MCF7 cells ranging from 50 to 5000 cells mL –1 and (B) their exosomes ranging from 100 to 4.0 × 10 4 exosomes μL –1 , according to NTA counting. In all cases, the cells and exosomes were lysed preconcentrated by IMS using antiCD81-MPs (▲) and antiCD326-MPs (●), followed by double-tagging RT-PCR on poly(dT)-MPs. The error bars show the standard deviation for n = 3.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Standard Deviation

    Panel A shows the control of the purified total exosome population obtained by ultracentrifugation (100,000 × g ) normalized according to the protein content (0.33 μg per assay). Panel B. Electrochemical genosensing of CD326+ exosomes from 1 mL of cell-free undiluted human serum (centrifuged at 10,000 × g ) based on immunomagnetic separation with antiCD326-MP and further GAPDH transcripts detection. The whole procedure is also shown in Figure . In all cases, serum-derived exosomes from healthy controls ( n = 10, pooled) and breast cancer ( n = 10, pooled) patients were processed. The error bars show the standard deviation for n = 3. The raw amperograms are also shown in Figure S4 (Supplementary Data). Created with BioRender.com .
    Figure Legend Snippet: Panel A shows the control of the purified total exosome population obtained by ultracentrifugation (100,000 × g ) normalized according to the protein content (0.33 μg per assay). Panel B. Electrochemical genosensing of CD326+ exosomes from 1 mL of cell-free undiluted human serum (centrifuged at 10,000 × g ) based on immunomagnetic separation with antiCD326-MP and further GAPDH transcripts detection. The whole procedure is also shown in Figure . In all cases, serum-derived exosomes from healthy controls ( n = 10, pooled) and breast cancer ( n = 10, pooled) patients were processed. The error bars show the standard deviation for n = 3. The raw amperograms are also shown in Figure S4 (Supplementary Data). Created with BioRender.com .

    Techniques Used: Purification, Immunomagnetic Separation, Derivative Assay, Standard Deviation



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    Image Search Results


    (A) Characterization by NTA of purified exosomes derived from the MCF7 breast cancer cell line. (B) Cryo-TEM images (i) and (ii) of purified exosomes at an acceleration voltage of 200 kV. (C) Confocal microscopy images and (D) flow cytometry study for the (i) MCF7 breast cancer cell line and (ii) their exosomes covalently immobilized on MPs. For confocal microscopy, DNA appears blue, magnetic particles in green color, while the exosome protein membrane in red color. For flow cytometry, in all cases, the negative controls (obtained with incubation with the secondary antimouse-Cy5) are shown in blue, while the positive CD326 and CD81 samples obtained with the incubation with the antiCD326 and antiCD81 primary antibodies, are shown in yellow and red, respectively. The negative control obtained with the exosomes-modified MPs without any labeling is shown in gray in panel D (ii).

    Journal: Analytical Chemistry

    Article Title: Electrochemical Genosensing of Overexpressed GAPDH Transcripts in Breast Cancer Exosomes

    doi: 10.1021/acs.analchem.2c04773

    Figure Lengend Snippet: (A) Characterization by NTA of purified exosomes derived from the MCF7 breast cancer cell line. (B) Cryo-TEM images (i) and (ii) of purified exosomes at an acceleration voltage of 200 kV. (C) Confocal microscopy images and (D) flow cytometry study for the (i) MCF7 breast cancer cell line and (ii) their exosomes covalently immobilized on MPs. For confocal microscopy, DNA appears blue, magnetic particles in green color, while the exosome protein membrane in red color. For flow cytometry, in all cases, the negative controls (obtained with incubation with the secondary antimouse-Cy5) are shown in blue, while the positive CD326 and CD81 samples obtained with the incubation with the antiCD326 and antiCD81 primary antibodies, are shown in yellow and red, respectively. The negative control obtained with the exosomes-modified MPs without any labeling is shown in gray in panel D (ii).

    Article Snippet: Tosyl-activated magnetic particles (MPs) (Dynabeads M450 Tosylactivated, ref. 14013), MPs modified with EpCAM antibody (antiCD326-MPs, Dynabeads Epithelial Enrich, ref. 16102), MPs modified with poly(dT) (polydT-MPs, Dynabeads Oligo(dT)25, ref. 61002), MPs modified with streptavidin (strep-MPs, Dynabeads MyOne Streptavidin T1, ref. 65601), mouse monoclonal antibody antiCD81 (ref. 10630D), and BCA protein assay kit (ref. 23225) were purchased from Thermo Fisher Scientific (MA, US).

    Techniques: Purification, Derivative Assay, Confocal Microscopy, Flow Cytometry, Incubation, Negative Control, Modification, Labeling

    Electrochemical genosensing of GAPDH transcripts from (A) MCF7 cells ranging from 50 to 5000 cells mL –1 and (B) their exosomes ranging from 100 to 4.0 × 10 4 exosomes μL –1 , according to NTA counting. In all cases, the cells and exosomes were lysed preconcentrated by IMS using antiCD81-MPs (▲) and antiCD326-MPs (●), followed by double-tagging RT-PCR on poly(dT)-MPs. The error bars show the standard deviation for n = 3.

    Journal: Analytical Chemistry

    Article Title: Electrochemical Genosensing of Overexpressed GAPDH Transcripts in Breast Cancer Exosomes

    doi: 10.1021/acs.analchem.2c04773

    Figure Lengend Snippet: Electrochemical genosensing of GAPDH transcripts from (A) MCF7 cells ranging from 50 to 5000 cells mL –1 and (B) their exosomes ranging from 100 to 4.0 × 10 4 exosomes μL –1 , according to NTA counting. In all cases, the cells and exosomes were lysed preconcentrated by IMS using antiCD81-MPs (▲) and antiCD326-MPs (●), followed by double-tagging RT-PCR on poly(dT)-MPs. The error bars show the standard deviation for n = 3.

    Article Snippet: Tosyl-activated magnetic particles (MPs) (Dynabeads M450 Tosylactivated, ref. 14013), MPs modified with EpCAM antibody (antiCD326-MPs, Dynabeads Epithelial Enrich, ref. 16102), MPs modified with poly(dT) (polydT-MPs, Dynabeads Oligo(dT)25, ref. 61002), MPs modified with streptavidin (strep-MPs, Dynabeads MyOne Streptavidin T1, ref. 65601), mouse monoclonal antibody antiCD81 (ref. 10630D), and BCA protein assay kit (ref. 23225) were purchased from Thermo Fisher Scientific (MA, US).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Standard Deviation

    Panel A shows the control of the purified total exosome population obtained by ultracentrifugation (100,000 × g ) normalized according to the protein content (0.33 μg per assay). Panel B. Electrochemical genosensing of CD326+ exosomes from 1 mL of cell-free undiluted human serum (centrifuged at 10,000 × g ) based on immunomagnetic separation with antiCD326-MP and further GAPDH transcripts detection. The whole procedure is also shown in Figure . In all cases, serum-derived exosomes from healthy controls ( n = 10, pooled) and breast cancer ( n = 10, pooled) patients were processed. The error bars show the standard deviation for n = 3. The raw amperograms are also shown in Figure S4 (Supplementary Data). Created with BioRender.com .

    Journal: Analytical Chemistry

    Article Title: Electrochemical Genosensing of Overexpressed GAPDH Transcripts in Breast Cancer Exosomes

    doi: 10.1021/acs.analchem.2c04773

    Figure Lengend Snippet: Panel A shows the control of the purified total exosome population obtained by ultracentrifugation (100,000 × g ) normalized according to the protein content (0.33 μg per assay). Panel B. Electrochemical genosensing of CD326+ exosomes from 1 mL of cell-free undiluted human serum (centrifuged at 10,000 × g ) based on immunomagnetic separation with antiCD326-MP and further GAPDH transcripts detection. The whole procedure is also shown in Figure . In all cases, serum-derived exosomes from healthy controls ( n = 10, pooled) and breast cancer ( n = 10, pooled) patients were processed. The error bars show the standard deviation for n = 3. The raw amperograms are also shown in Figure S4 (Supplementary Data). Created with BioRender.com .

    Article Snippet: Tosyl-activated magnetic particles (MPs) (Dynabeads M450 Tosylactivated, ref. 14013), MPs modified with EpCAM antibody (antiCD326-MPs, Dynabeads Epithelial Enrich, ref. 16102), MPs modified with poly(dT) (polydT-MPs, Dynabeads Oligo(dT)25, ref. 61002), MPs modified with streptavidin (strep-MPs, Dynabeads MyOne Streptavidin T1, ref. 65601), mouse monoclonal antibody antiCD81 (ref. 10630D), and BCA protein assay kit (ref. 23225) were purchased from Thermo Fisher Scientific (MA, US).

    Techniques: Purification, Immunomagnetic Separation, Derivative Assay, Standard Deviation